Human Mammary Cell Types Available For Distribution
Cells are sent frozen (dry ice) in ampoules containing 5 x 10-5 or 1 x 10-6 cells. Under special circumstances, we can arrange to send growing cells in flasks. (see "REVIEW section VII." for more information on cell shipments)
FINITE LIFESPAN HMEC (section I.)
1. Reduction mammoplasty derived HMEC grown in serum free (MCDB 170 like) medium
Limited quantities of post-selection cells are available around passages 7-10 that senesce around passages 15-25 (about 3 population doublings per passage). These cells are routinely available from women of different ages. I most commonly distribute cells from specimens 184, 48R, 239 and 161 (see section I.C. figure 1).2. Reduction mammoplasty derived HMEC grown in a serum containing (MM like) medium
Limited quantities of cells are available from several individuals. Cells are supplied at 2nd or 3rd passage; most growth ceases around passages 4-5. They contain a greater range of phenotypes than the post-selection MCDB 170 grown cells. I most commonly distribute cells from specimens 184, 48, and 161.3. Cells from non reduction mammoplasty tissues (i.e., mastectomies, benign tumors, gynecomastias)
Limited quantities are available. Cells grown from tumor tissues in MCDB 170 are not reflective of most tumor cells. Talk with me directly about these cells.FINITE LIFESPAN HUMAN MAMMARY FIBROBLAST CELLS (section I.)
We have available stocks of fibroblast cells from several reduction mammoplasty specimens for which HMEC are available, particularly specimens 184, 48, and 161. These cells are grown in a serum containing medium. In theory, fibroblast stocks can be obtained from any of our specimens, including the mastectomy derived tissues, but we have not grown up stocks to distribute from more than a few. Frozen cells are available around passages 4-6, and they senesce around passages 10-20 (2-3 population doublings per passage).
PRIMARY TISSUES (section I. AB.)
We have very limited quantities of frozen organoids, and are therefore very reluctant to distribute any of this material, but will make exceptions for specific studies. More primary tissue is available from reduction mammoplasties than mastectomies. Talk with me directly about these cells.
IMMORTALLY TRANSFORMED CELL LINES (section II. & III.)
1. 184A1
This cell line of indefinite lifespan is available for distribution. For some purposes, it may be preferable to have early or later passage cells. Clonal isolates are also available. These cells are wild type for p53, and RB, and are not anchorage independent or tumorigenic.
More limited quantities of cells are available which have been selected for loss of specific nutritional requirements.
184A1 retrovirally infected with either the HPV16 -E6, -E7, SV40T, or E1A genes are also available. Talk with me directly about these cells.
2. 184B5
This cell line of indefinite lifespan is available for distribution. For some purposes, it may be preferable to have early or later passage cells. Clonal isolates are also available. These cells are wild type for p53, and RB, and are not anchorage independent or tumorigenic.
More limited quantities of cells are available which have been selected for loss of specific nutritional requirements.
More limited quantities are available of cells which have been transfected with erbB-2 and rendered anchorage independent..
3. 184AA4
This is a newly derived cell line of indefinite lifespan derived from the same extended life precursor population as 184A1. These cells are wild type for p53, and RB, and have not been tested for anchorage independence or tumorigenicity.
4. 184AA2 and 184AA3
These are newly derived cell lines of indefinite lifespan derived from the same extended life precursor population as 184A1. These cells are p53-/- and wild type for RB, and have not been tested for anchorage independence or tumorigenicity.
5. 184-E6
This is specimen 184 post-selection cells retrovirally infected with the HPV16 E6 gene and rendered immortally transformed. Talk with me directly about these cells.
CARCINOGEN EXPOSED EXTENDED LIFE CULTURES (section II.A. and figure 3)
Very limited quantities of benzo(a)pyrene treated specimen 184 extended life cultures, including the extended life precursors of the lines 184A1 (184Aa) and 184B5 (184Be), are available. Talk with me directly about these cells.
Email:mrstampfer@lbl.gov__|__ Back