2-HMT-3522 density

1- volume

4 wells chamber slides = 1.7 cm²

4 wells = 2 cm²

35 mm = 9.62 cm²

T25 = 25 cm²

60 mm = 26.26 cm² = 3 X 35mm

100 mm = 78.54 cm² = 8 X 35mm

T75 = 75 cm² = 7.8 X 35 mm or 3X T25

	Total volume of medium (half with matrigel if OT culture)	Volume of matrigel for On top + %drip culture	Volume of matrigel for 3d culture
4 wells Chamber slides	0.3-0.4 ml	60 ml / 2 to 10% FV (20ml)
4 wells	0.5ml		0.3 ml
35 mm	1 - 2 ml	0.5 ml / 2 to 10% FV	1.2 ml
60 mm	3 – 5 ml	1.5 ml / 2 to 10% FV	3.6 ml
T25	4 - 5 ml	-	-
T75	10 ml	-	-

2- density

S1 cells: 2X10⁴ cells/cm²

T4-2 cells: 1X10⁴ cells/cm²

a- S1 cells

	Plastic (2d)	On top (OT)	Inside (3d)
4 Chamber slides	35000 cells per well 40000 cells per well	40000 cells per well 50000 cells per well
35 mm 9.62 cm^2	0.2 M to 0.25 M	0.8 M 0.3 M	0.8M/ ml matrigel
60 mm or T25 26.26 cm^2	0.5 to 0.65 M	2.4 M 0.9 M
100 mm or T 75 ~ 75 cm^2	1.5M to 1.75

b- T4-2 cells

	Plastic (2d)	On top (OT)	Inside (3d)
4 Chamber slides	17000 cells per well	0.04 M
35 mm 9.62 cm^2	0.08 M	0.2 M	0.6M/ ml matrigel
60 mm or T25 26.26 cm^2	0.25 M	0.6 M
100 mm or T 75 ~ 75 cm^2	0.75 M	1.6 M