Centrifugation is performed in screw cap or pressure
seal tubes/bottles which have been secondarily sealed with Parafilm
(or equivalent). If there has been any possibility of leakage,
the inner walls of the centrifuge chamber and the rotor should be
immediately decontaminated. In the event that there are no
safety centrifuge tubes or carriers available, the centrifuge can
be placed in a biosafety cabinet. It is important to place
the centrifuge away from the front of the cabinet opening.
Class II Cabinets are
installed and certified in accordance with National Sanitation Foundation
Exhaust air volume (CFM) are to be maintained at design
condition, regardless of changes in filter resistance. In-line
damper with manometer across filter and damper or equivalent monitoring
system to be used.
Laboratories with supply air will have the supply air
volume (CFM) controlled so as to assure inflow of air to laboratory
from adjacent areas.
Ventilation systems are to be monitored on an annual
basis by the EHS and records kept indicating airflow, filter resistance,
and other pertinent data kept.
you have finished Section II. Go to next section.