|Secrets of the wine cellar: the genome of a wine-making microbe|
|Lynn Yarris, firstname.lastname@example.org|
All who enjoy the taste of wine stand to benefit from the latest round of genome sequencing at the U.S. Department of Energy's Joint Genome Institute. JGI researchers have completed the sequencing of Oenococcus oeni, a lactic-acid fermenting bacterium that plays a critical role in de-acidifying wine after alcoholic fermentation has been completed.
Most wine lovers know that yeast is used to ferment the sugar in the grape "must" (the mixture of skins, pulp, seeds, and juice) into alcohol. Less familiar is the need in many wines for a secondary fermentation converting harsh-tasting malic acid into lactic acid, a much softer-tasting acid so named for its presence in milk. Malolactic fermentation contributes to flavor, aroma, and texture. For some wines, it can play a role in spoilage and taint.
The yeast species most commonly used in the primary fermentation of wine, Saccharomyces cerevisiae, does not metabolize malic acid. In many wines, the malolactic fermentation will be carried out spontaneously by lactic acid bacteria, in particular, Oenococcus oeni, which is unusually tolerant of the wine's acid and ethanol concentrations. However, this species of bacteria grows slowly, and if the malolactic fermentation process is not timed to start immediately after the alcohol fermentation, its performance can be sluggish.
"Sequencing the genome of Oenococcus oeni will impact the wine industry for many years to come," says David Mills, a viticulture and enology professor at the University of California at Davis. "Moreover, access to the aggregate sequences will completely alter the scientific perspective on lactic acid bacteria."
Mills is coleader, along with Utah State University professor Bart Weimer, of a nationwide, multi-institute scientific collaboration called the Lactic Acid Bacteria Genome Consortium (LABGC). Through the consortium, Mills and Weimer developed a proposal for the Oenococcus oeni genome to be sequenced at JGI under the U.S. Department of Energy's Microbial Genome Program.
"Our goal is to use this sequence to advance genomic study on the lactic acid bacteria and other food fermentation microbes," says Mills. "We want to gain a more comprehensive understanding of the microbes' genetics and physiology, particularly in relation to their role in fermented foods and beverages. We also want to identify and enhance the beneficial roles of these bacteria in food preservation and safety, and in the health of humans and animals."
JGI is a collaboration of Lawrence Berkeley, Lawrence Livermore, and Los Alamos National Laboratories, funded by DOE's Office of Biological and Environmental Research. Its headquarters in Walnut Creek, California, houses one of the nation's fastest and most powerful genome sequencing operations. JGI researchers completed their sequencing of the Oenococcus oeni genome within a year.
"Straight sequencing of an individual microbe can be done in a day with our capacity here," explains Kristen Kadner, a biologist with Berkeley Lab's Genomics Division who helped coordinate JGI's lactic acid bacteria effort. "However, the process is stretched out and spaced between other types of microbes in order to help prevent contamination. Once the initial sequencing phase is complete, we pass the data through a preliminary quality control analysis before the rest of the genome is taken to full 10-X sequencing," meaning that each genome is sequenced a total of 10 times to minimize error rate and maximize the quality of the data.
Mills expects JGI's completion of the Oenococcus oeni genome sequence to be comparable to the completion of the genome sequence for yeast in 1996 in terms of beneficial impact. The data should help scientists gain a better understanding of malolactic fermentation and the interactions between lactic acid bacteria and yeast. This in turn should not only boost scientific efforts to improve wine quality, but should also benefit dairy fermentations and the burgeoning field of probiotics -- improving human health and immunity through the ingesting of certain bacteria.
JGI sequencing data is available to the public with no restrictions. Mills and his UC Davis research group will fill in any gaps in the JGI-generated sequence of the Oenococcus oeni genome with funding from the American Vineyard Foundation and the California Competitive Grants Program for Research in Viticulture and Enology.
"With this genetic roadmap in hand, the ability of scientists to study the genetics, ecology and physiology of lactic acid bacteria in wine and other fermented products will be amplified tremendously," Mills says.