Long before PCR achieved mainstream recognition, however, LBNL researchers used PCR to help map and sequence the three billion letters of the human genetic code.
Now, engineers in LBNL's Human Genome Center instrumentation group, working closely with Lab biologists, have developed an improved type of PCR apparatus that can perform the required steps in less than half the time. Chris Martin of the HGC's sequencing group says the next, even faster, version of the new machine, now under development, could lead to a total robotic automation of the process.
PCR involves three steps (see article on page 2), each of which must be performed at a specific temperature. To be most effective, the temperature changes should be as rapid as possible. In conventional PCR equipment, an array of tubes or vials holding samples of DNA is placed in a metal block, and the temperature of the samples is controlled by heating and cooling the block.
In the new apparatus, known as the rapid thermal cycler, the temperature is controlled with circulating water, which results in faster temperature changes, more samples processed per hour, and an improved signal-to-noise ratio in the data. The new design reduces sample preparation and handling procedures by about 40 minutes per array.
In each step of the PCR process, precise regulation and rapid switching of temperature is crucial. In the prototype, designed by Tony Hansen, the DNA samples are held in a standard plastic microtiter plate half-submerged in water fed from three separate tanks, maintained at 95, 72, and 55 degrees centigrade. The user selects one of several preprogrammed timing protocols, and a system of computer-controlled valves switches the water for each step. This model has been operating for about 18 months in the HGC laboratories in Bldg. 74.
An improved version of the rapid thermal cycler is in the final stages of development. In this model, designed by Kanchi Karunaratni, the heating tanks, interlocks, and valves are all directed by a sophisticated process controller (similar to the computers that run automated factory assembly lines). This results in more precise regulation of temperature and more flexible switching of the valves. The new model is also smaller and more energy efficient. Three models of this version are being built, with the first due to go on line at HGC this summer.
In addition to Hansen and Karunaratni, LBNL engineers and technicians involved in the design of the rapid thermal cycler include Dave Wilson, Davey Hudson, Charlie Reiter, and Don Uber (software). Joe Jaklevic heads the HGC instrumentation group.
In the first step, denaturing, the test tube is heated close to boiling for a few seconds. This causes the double-stranded DNA to separate into two single strands. The primers bind to the exposed single strands at places where the sequence of primer bases is complementary to that of the DNA.
The second step is annealing. The temperature of the test tube is lowered to about 55 degrees centigrade for a few seconds, causing the primers to bind permanently to their sites on the single-stranded DNA. The DNA of interest is now single-stranded along most of its length, with a few small double-stranded areas where primers have aligned themselves.
The third step is extending. The temperature is raised to about 72 degrees centigrade for about a minute, which causes the polymerase protein to go to work. It moves along the single-stranded portion of the DNA, beginning at a primer, and creates a second strand of new DNA to match the first. After extension, the DNA of interest is double-stranded again, and the number of strands bearing the sequence of interest has been doubled.
The three steps are repeated about 30 times, resulting in an exponential increase of up to a billion-fold of the DNA of interest. A fragment of DNA that accounted for one part in three million in the original sample now fills the whole test tube.
While voting to end the use of ethnicity and gender in student admissions and in hiring and contracting, the Regents issued a companion statement declaring California's diversity as an asset. Shank emphasized the value of such diversity at LBNL.
"We continue to believe that our strengths derive from the fair and equitable treatment of employees and the stimulating variety of individuals who make up our community," Shank said. "We respect diversity in its many forms and will sustain our support for programs and policies which protect individual rights while preserving the quality of our multicultural environment."
In a statement released on Monday following the Regents' decision, UC President Jack Peltason noted that a provision in the resolution dealing with business practices clearly states that the University will continue to comply with any requirements necessary to maintain its eligibility for federal and state funds. "Few significant changes are likely," he said, "because UC's employment and contracting programs are governed by state and federal laws, regulations, executive orders, and the U.S. Constitution, and our practices historically have been and will continue to be in compliance with those various laws and requirements."
The Regents voted 14-10 to bar the use of race, religion, sex, color, ethnicity or national origin as criteria for University student eligibility and admission, effective Jan. 1, 1997. In a separate 15-10 vote, Regents also eliminated the use of ethnicity and gender in UC's hiring and business practices, effective Jan. 1, 1996. The two resolutions approved by Regents were proposed by Regent Ward Connerly.
The meeting in San Francisco was attended by 800 members of the public and about 300 members of the press. During 12 hours of public comment and debate about the issue, about 30 public officials and about 30 members of the public--including the Rev. Jesse Jackson, Assemblyman Willie Brown Jr. and Gov. Pete Wilson--spoke on the affirmative action issue.
UC President Peltason, who had urged support for affirmative action policies, said he had hoped for a different outcome. "I want to ask the members of the University community, whatever your views on the matter, to keep in mind that our goal has not changed," he told the press after the vote at UC San Francisco. "The only thing that has changed is the means we can take to achieve our goal."
In a joint statement, Peltason and the nine UC chancellors said: "This will unfortunately make it more difficult for our campuses to achieve the diversity that is essential for the future excellence of the university and the stability and welfare of our society. However, we pledge to continue our efforts to serve all populations in California, working within the new guidelines of economic and social disadvantage, and in conformance with state and federal mandates. We pledge to retain those elements of affirmative action that have proven of such great value to our institution, including open employment searches."
Regent Connerly said this week, "We do not want to be perceived as turning our back on achieving diversity. We're saying that we're totally committed to diversity and inclusion, and we're going to make sure everyone has equal opportunity. The only question is how to do it. If what we're doing conflicts with federal laws and programs, then the federal law supersedes. We do not want to be in a stand-off game with UC and the federal government."
"Provost (Walter) Massey joins me in wishing you continued success with LBNL," Peltason wrote. "We understand the recent years have sometimes been difficult ones for the laboratories, and changing times in DOE and the Congress promise more challenges ahead. Nonetheless, we are confident that you will lead the Laboratory to new scientific excellence. You have an outstanding staff, and we trust that your leadership will see them through the changes ahead."
As part of UC management procedures, all chancellors and Department of Energy laboratory directors undergo performance reviews every five years. The results of those reviews are personnel matters and therefore considered confidential.
Shank's evaluation began in December and included invited comments from lab employees. Two closed meetings were held by a special review board in December and January for purposes of gathering input, and employees were encouraged to submit additional comments in writing.
John Armstrong, retired vice president of IBM, served as chairman of the review panel. Other members included Patricia Buffer, dean of the UC Berkeley School of Public Health; Boyce McDaniel, professor emeritus of Cornell University; Christopher McKee of the UCB Space Sciences Lab; Thomas Page, San Diego Gas and Electric; Kumar Pael, UCLA vice chancellor for research; and Lucy Shapiro of the Stanford Medical Center.
From time to time, the attorneys and patent agents in LBNL's Patent Department receive questions from individual researchers, the answers to which may be of interest to others in the Laboratory. If you have questions for the Patent Department, call X7058.
Q: What is the current situation regarding patenting of computer software in the United States?
A: Until relatively recently, it was extremely difficult to obtain software patents in the United States. In the last two years, however, there have been more than 10,000 software patents issued by the U.S. Patent Office. The ten most frequent art categories are image processing, network/communications, operating systems, process/numerical control, graphics, medical/health care, engineering, automobile/transportation, graphical user interface, and signal processing.
In 1994, there were 396 software patents issued to IBM, 189 to Hitachi, 107 to DEC, 107 to Xerox and Fuji Xerox, 107 to Toshiba, 97 to Hewlett-Packard, 82 to Fujitsu, 70 to Canon Kabushiki Kaisha, 68 to Motorola, and 68 to Matsushita Electric Industrial. It should be noted that five out of the top ten corporations receiving software patents are Japanese, or Japanese-owned.
Many researchers at LBNL have developed software programs which may be commercially significant and patentable, but only a few of these programs have been reported to the Patent Department. Contract 98 requires that all computer programs be reported to DOE via the Patent Department. To assist in reporting, the Patent Department has now prepared a new Software Disclosure Form, which can be obtained electronically (Word 5.1) from the Patent Department public folder Zone B12; name Patent Department, Karen Drew.
LBNL researchers are encouraged to submit their software programs to the Patent Department for consideration of either copyrighting or patenting, as may be most appropriate.
Out-of-this-world exhibits currently on display include "Electric Space: Exploring Our Plasma Universe," featuring activities about the electric and magnetic forces that create phenomena such as the Northern Lights aurora; the Holt Planetarium, which lets visitors participate in observations and experiments about the heavens; and Saturday Night Stargazing, where visitors can view the moon, planets, stars, and galaxies from the LHS plaza (8:30 to 11 p.m.).
Call LHS at 642-5134 for more information.
Reception listings include ads appearing in the Currents flea market as well as ads from non-employees. To post a housing ad in Currents, send via e-mail to fleamarket@lbl.gov, or fax to X6641.
Gilbert received a $100 savings bond for 15 years of accident-free driving. Bell received a $75 bond and Miller a $50 bond for 10 and 5 years of safe driving, respectively. Other drivers who completed 1994 with no preventable accidents were also awarded certificates. The awards were presented by Fred Lothrop of Bus and Fleet Services, and Dave Saucer, who oversees the Transportation Office.
The awards were handed out at the 40th Annual Safe Drivers Awards luncheon, held in the cafeteria on June 15 to honor all LBNL bus and truck drivers for their years of driving with no preventable accidents. Saving bonds were given to drivers for every fifth year of safe driving completed. To be eligible, driving must be at least 50 percent of an employee's job.
The UC Office of the President is working with the Centers for Disease Control (CDC) and the State Department of Health Services to prepare information and guidelines about Hantavirus risk reduction, precautions and clean up. Here are some answers to commonly asked questions about the virus:
What is a Hantavirus?
Hantaviruses are a family of four previously identified viruses, found in rodents, that have caused serious health problems in other parts of the world (mainly the Far East and Scandinavia).
How is the virus transmitted?
The virus is believed to be principally carried by a common rodent, the deer mouse, which is found throughout North America and in every California county. Infected rodents shed live virus in saliva, feces and urine. Humans are infected when they encounter and inhale aerosolized microscopic particles that contain dried rodent urine or feces.
How dangerous is this virus?
This strain appears to be extremely dangerous to those who are infected with it. While dangerous to individuals, its means of transmission is so unusual that most people are very unlikely to encounter the virus, thus, it poses little threat to the general California population.
The CDC recommends that all persons minimize their contact with deer mice and other rodents. LBNL Health Services has information to help you protect yourself from infection by recognizing risks and taking precautions.
Hikers and campers, and those doing research with rodents or working at remote locations are encouraged to avail themselves of this information. Call X6266 or stop by Health Services (Bldg. 26) to pick up this information.
Date Demonstration Time Location 8/1 LBNL Library & MELVYL Catalogs 1:30 p.m. 62-339 8/10 LBNL Library & MELVYL Catalogs 11 a.m. 50-134 8/15 LBNL Library & MELVYL Catalogs 3 p.m. 62-339 8/16 Thomas' Register on CD-ROM 11 a.m. 90P 8/22 TULIP 3 p.m. 62-339 8/24 TULIP 11 a.m. 50-134 8/30 LBNL Library & MELVYL Catalogs 11 a.m. 90P 8/31 LBNL Library & MELVYL Catalogs 11 a.m. 50-134
Date Course Time Place 8/9 Fire Extinguisher Use (EHS 530) 10-11:30 a.m. 48-109 8/9 Recertification for Crane/Hoist (EHS 216) 8 a.m. - noon 70-191 8/15 Introduction to EH&S (EHS 10) 9-11:30 a.m. 66 Aud. 8/15 Lockout/Tagout Training (EHS 256) 9-11:30 a.m. 51-201 8/16 Blood Biosafety Training (EHS 735) 9-10:30 a.m. 4-102 8/17 Adult CPR (EHS 123) 9 a.m. - noon 48-109 8/22 Earthquake Safety (EHS 135) 10-11:30 a.m. 48-109 8/22 Forklift Recertification (EHS 226) 10-11 a.m. 51-201 8/23 First Aid (EHS 116) 8 a.m. - noon 48-109 8/23&25 Radiation Protection-Radiological Worker I (EHS 430) - both days 8 a.m. - noon 51-201 8/24 Laser Safety (EHS 280) 9:30-11:45 a.m. 51-201 8/29 Pressure Safety/Compressed Gases (EHS 230) 8 a.m. - noon 51-201 8/29 Chemical Hygiene and Safety Training (EHS 348) 1-4:30 p.m. 51-201 8/30 Med/Biohazardous Waste Training (EHS 730) 10-11:30 a.m. 66-316Pre-registration is required for all courses except Introduction to Environment, Health & Safety. Call X6554 to register for CPR, First Aid, Fire Extinguisher Use, Earthquake Safety, and Building Emergency Team Training. Send a fax with your name, employee number, extension, and mail stop to X7209 to pre-register for all other courses (or call X6612).
31 m o n d a y
OPEN CALENDAR
1 t u e s d a y
LBNL LIBRARY DATABASE TRAINING
LBNL Library & MELVYL Catalogs, at 1:30 p.m.in Bldg. 62-339.
SURFACE SCIENCE AND CATALYSIS SCIENCE SEMINARS
"Aspects of Local Probe Microscopy: From Tunneling to Force Sensing" will be presented by Urs Durig of the IBM Research Division, Zurich, Switzerland, at 1:30 p.m. in the Bldg. 66 Auditorium.
2 w e d n e s d a y
OPEN CALENDAR
3 t h u r s d a y
OPEN CALENDAR
4 f r i d a y
CENTER FOR BEAM PHYSICS SEMINAR
"Entropy and Emittance of Particle and Photon Beams" will be presented by Kwang-Je Kim of LBNL at 10:30 a.m. in the Bldg. 71 conference room.
THE CENTER FOR ENVIRONMENTAL BIOTECHNOLOGY SEMINAR
"Unsaturated Zone Gas-phase VOC Biodegradation; The Importance of Water Potential" will be presented by Patricia Holden of UCB at noon in Bldg. 50A-5132.
UPTE - LBNL LOCAL 184 MONTHLY MEMBERSHIP MEETING
Noon - 1 p.m., lower cafeteria conference room, all Technical Employees and Research Associates are invited to attend, especially non-members. Q & A on the RIF and changes in the RPM which will impact employees.
7 m o n d a y
BODYWORKS
Noon - 1 p.m, meeting in the lower cafeteria.
8 t u e s d a y
OPEN CALENDAR
9 w e d n e s d a y
EH&S CLASSES
Recertification for Crane/Hoist (EHS 216), 8 a.m. - noon, Bldg. 70-191; pre-registration required, X6612.
Fire Extinguisher Use (EHS 530), 10 - 11:30 a.m., Bldg. 48-109; pre-registration required, X6554.
NUCLEAR SCIENCE DIVISION COLLOQUIA
"A Review of Recent Results from the SPS Heavy Ion Program" will be presented by Barbara Jacak of the Las Alamos National Lab at 4 p.m. in Bldg. 70A-3377.
10 t h u r s d a y
LBNL LIBRARY DATABASE TRAINING
LBNL Library & MELVYL Catalogs, 11 a.m., Bldg. 50-134
AFRICAN AMERICAN EMPLOYEES ASSOCIATION
General Body Meeting, noon - 1 p.m., Bldg. 90-1099
11 f r i d a y
OPEN CALENDAR
Results of July 26 CAMShafts 14
Animals 5Rated X 14
Astros 4Budget Cuts 11
CAMShafts 10Environ-mets 13
Off the Hill 6Sudz 1
Budget Cuts 0Off the Hill 2
Ball Park Estimates 13Standings as of July 19 W-L Ball Park Estimates 7-1 Rated X 8-2 Environ-mets 7-2 Astros 6-4 Sudz 3-7 CAMShafts 6-4 Animals 2-6 Budget Cuts 2-8 Off the Hill 1-8
A location of particular concern is the Par Course, where a large portion of the broom is located and where cut broom is drying prior to the controlled burn in September; please do not use the pathways that traverse this area. As work continues through the summer, detours will be posted in other areas as well. With your cooperation, this job can be completed safely and with a minimum of disruption to the Laboratory.
Early Bird Peach pancakes w/coffee -- $2.05
Sadie's Grill Meatball sub w/fries -- $3.25
Passports South of the Border -- (al a carte)
Soup Corn chowder -- $1.35 & $1.95
Bistro fare Chinese chicken salad* -- 3.95
tuesday
Early Bird Berry French toast w/coffee -- $2.05
Sadie's Grill Tuna melt w/fries -- $3.05
Passports South of the Border -- (al a carte)
Soup Mushroom barley* -- 35 & $1.95
Bistro fare Glazed ham, macaroni & cheese, green beans -- $3.95
wednesday
Early Bird Eggs Benedict w/coffee -- $2.95
Sadie's Grill Hot pastrami on rye w/fries -- $3.05
Passports South of the Border -- (al a carte)
Soup Split pea & ham -- $1.35 & $1.95
Bistro fare Green onion beef stirfry w/noodles* -- $3.95
thursday
Early Bird Blueberry pancakes w/coffee -- $2.95
Sadie's Grill Chicken breast on a bun w/fries -- $3.75
Passports South of the Border -- (al a carte)
Soup Creamy clam chowder -- $1.35 & $1.95
Bistro fare Delta catfish, hush puppies, greens -- $3.95
friday
Early Bird Ham scramble w/coffee -- $2.60
Sadie's Grill Grilled Reuben w/fries -- $3.95
Soup Beef noodle -- $1.35 & $1.95
Bistro fare Pasta Piatti (pasta & grilled veggies)* -- $3.95
*Denotes recipe lower in fat, calories & cholesterol
AUTOS/SUPPLIES
'69 CAMARO Z28, silver/black, Chevy 350, orig. Holley, hi-rise manifold, headers, hurst, 12-bolt posi rear, 8k mi., great cond., $11,900. (408)356-1936
'82 MAZDA 626, stick shift, 125K mi., new brake shoes,
a/c, runs great,
$1650. Fabrice, X7960, 664-2997, Laurent, (415)859-4966
'84 BMW 733, exc. running cond., needs some paint, $6500. (415)381-9814
'85 PORSCHE 944, red w/black int., sunrf., low mi., $5500/b.o. Russ, 339-9812
'86 FORD Escort wgn, 65K mi., a/t, a/c, p/s, p/b, snow chains, recent tune-up, $2200. John, 601-0730 (before 10 p.m.)
'87 CELICA GTS, 2-dr hatchbk, 5-spd, 2-tone gray, loaded, all pwr, new (used) engine incl. warranty, new brakes, struts, clutch, year old tires, exc. cond., $5500/b.o. Ervette, X6135
'87 PLYMOUTH Sundance, 4-dr, 88K mi., new tires, shocks, struts., 5-spd, runs great, $2900. Joy, (415) 456-2966
'87 VOLVO 240 wgn, stick, a/c, body 66K mi., motor & clutch 24K, tires 15K, great cond., $9K/b.o. Lee Schipper, X5057, 527-5821
'88 DODGE Dakota truck, white w/camper shell, clean, runs gd., $3950/b.o. John X4631
'88 MAZDA MX6, $5,000. X7176
'89 TOYOTA 4x4 pickup w/camper shell, $6K/b.o. X7176
'91 FORD Escort wgn, a/c, a/t, AM/FM/cass. stereo, clean, new trans., 1 owner, 80K mi., $6K. Jane, X6731
'93 SATURN SC2, silver, ABS, CD player, loaded, 42K mi., $12.5K. John, 601-0730 (before 10 p.m.)
TIRES, (2) Michelin XAS 165 HR 14 (for Alfa), like new. Jacob, X4606
TRAVEL TRAILER, '89 Alpenlite 5th wheel, 29'11", rear kitchen, sand color, exc. cond., 4 new tires, $18K. Richard, X6015, 689-1255
TICKETS
FOOTBALL, 49ers/Raider rights/season tickets, 50-yd. line seats. John, (415)924-3210
WANTED
HOUSE TO RENT on lake w/sm. sailboat, 8/20-27 or other times. John McCarthy, X5307, 841-7875
MAC POWERBOOK, used, needed for lab business. Jacob, X4606
MISCELLANEOUS
BABY STROLLER, Gerry, great cond., $50. 527-0693
BICYCLE, Nishiki men's 10-spd, exc. cond., extras incl. woman's seat, best offer. 524-1140 (10 a.m.-8 p.m.)
BIKE TRAILER, Burley, '90 model, seats 2 kids, up to 100 lbs., screen cover & rain fly incl., $225. 268-0674
BOOKBAG, blond leather, 4 inner compartments, adj. shoulder strap, exc. cond. (almost brand new), orig. $160, will sell for $100. Melissa, X7849
CAMERA LENS, Tamron 28 - 200mm AF, Nikon mount, B&W filter, close-up attach, mint cond., bought all for $350, asking $275. Peter, X5816
CROCK POT, elec., 3.5 qt., never used, $15; baseboard heater, Intertherm, oil/elec., 6' long, $60. 524-9473
DESK, walnut, $100; futon w/frame, $100; 3 bookcases, $25 ea.; sm. table, $20; night stand, $10; 2 desk lamps, $5 ea.; coffee maker, sm. rice cooker, mixer, $5 ea. Wolfgang, X4134, 528-8958
EXERCISE STEPPER, Prosport Fitness, dual action, adjust. shocks, w/monitor for time, dist., cals. & counter, like new, $125. Jim, X5450
MICROWAVE, Sanyo, $35. Radim, X5040
MOVING SALE, refrig., washer & dryer, sewing machine & sm. appliances. Eileen, 784-3702, 793-3118 (eve.)
SAILBOARD,'93 Fanatic Mega Ray 282, 9'-3", fast, exc. cond., w/2 blade fins, $375; North Infinity 4.3, $95. X6797, 236-4347
SAILBOAT, J24, exc. cond., $11,110. (415)381-9814
SOFA & CHAIR, antique red, hand carved, circa 1920s, $1300/b.o.; 1/4 carat diamond wed. ring w/10 small diamonds, $1100; queen sz. futon w/frame, $75; VCR w/remote, $90. Greg 339-0509
STEREO AUDIO RACK, Sansui, $25; drafting table, $30. 233-0734
SURVEYING INSTRUMENT, Berger speed series model 190B, gd. cond., $100/b.o. Kelly, X5468 (11 a.m. - 2 p.m.)
TELEVISION, Zenith 25" fl. model, exc. picture, keyboard entry tuning, approx.. sz. 2'x4'x2.5'H, $125/b.o. 235-3983
HOUSING
ALBANY, 1-bdrm in 3-bdrm, 2-bth apt, nr UC Village, avail.
8/19,
$270/mo. + 1/3 utils. + $100 dep. Mark, X4427
BERKELEY, 2-bdrm top sunny flat, lg. deck, green yd., nr UC/BART/LBNL shuttle area, furn., $1200/mo., lease. Mr. Bogdis, 540-8421
BERKELEY, sunny, unfurn. 2nd fl 1+ bdrm in Victorian 4-plex, wall-to-wall carpet, porch, no laundry or off-st. parking, Grant & Derby, short-term OK, $629/mo. + dep. + utils. 548-6974
BERKELEY, rm avail. in 2-bdrm apt. nr Dwight Way at Milvia, top fl., park sp. avail., quiet, no smoking, no pets, walk to UC, trans., avail. 8/15, $378/mo. + elec. & phone. Suzanne, X5012, 548-5074
NO. BERKELEY, 3-bdrm, 1-1/3 bth home, hardwd flrs, frpls, yd, washer/dryer, unfurn., sunny, quiet, nr trans. & shops, 1 mi. from UCB, avail. 8/2, $1500/mo. Guy, X4703, 548-0120
EL CERRITO, furn. 2-bdrm, 1-bth house, dinning rm, family rm, frpl, carpet, yd, walk to BART/Plaza, $1200/mo. + util. X7961, 232-7433
EL CERRITO, unfurn. 3-bdrm house, no pets, no smoking, avail. 8/5, $1100/mo. Mrs. Kim, 524-4199
EL CERRITO, new 3-bdrm, 2-bth house, dinning rm, family rm, sitting rm, frpl, carpet, yd, partial bay view, $1600/mo.+$50/mo. gardener. Hashem Akbari, 299-0560
EL CERRITO, sublet, 1-bdrm apt, 2 blks from Plaza, BART, quiet, detached unit, deck, washer/dryer, sunny, hardwd flrs, storage space, avail. 8/1 to 12/31, no smoking, pets, $650/mo. + utils. 526-8176
OAKLAND, 2-bdrm upstairs apt in classic brn-shingle house, Grand-Lake area, walk to Lake Merritt, Grand Ave., BART, Piedmont Ave. pref. quiet, non-smoker(s), reasonable utils. are incl., $650. 268-0674
NO. OAKLAND, Temescal area, 1200 sq. ft., 1-bdrm flat, sep. living & dining rms, lg. kitchen, dishwasher, laundry hook-ups, walk-in closet, frpl, hardwd flrs, yard, avail. late Aug., $800/mo. + util. Tom, 601-0574 (eve./wkend)
SAN LEANDRO, 1-bdrm apt, water/garbage pd., off st. parking, $525/mo. + dep. Timothy, 530-3033
WANTED: Apt for visiting academic & child from late Aug. '95 to late Feb. '96, nr UCB & bus/BART. mdonn@vuw.ac.nz or +64 4 802 6221
WANTED: 2-bdrm or lg. 1-bdrm apt, nr UCB or LBNL shuttle. X6031
WANTED: Visiting prof. seeks quiet studio or 1-bdrm apt, nr UCB, from Aug. to end of Nov. Joerg, 601-1626
WANTED: 1-bdrm apt for visiting prof. & wife, 9/10-10/7/95, allergic to pets. Luanne, X5853
WANTED: Visiting postdoc & wife w/5 yr. old daughter seek 2-bdrm apt or house in or nr Berkeley, end of Sept. through Dec. X5761, 848-538
WANTED: German sci. seeks 1-bdrm apt. w/balcony (or garden access) in nice area from 10/95 to 6/96. Martin, X5738
REAL ESTATE
SO. BERKELEY, Benvenue nr Ashby, 3+2 bdrm, 2-1/2 bth, brn. shingle, Craftsman style, lg. rms., high beamed ceilings, 2 frpls, $369K. Guy, X4703, 548-0120
EL CERRITO, downslope, 0.45 acre bordering Wildcat Reg. Park, great geol. report OMC, $140K. Mary, 339-8432
EL CERRITO HILLS, 2-bdrm, 1-bth home, move-in cond, bay view, almost new carpets, dining rm, hardwd flrs, frpl, lg. terraced backyd w/drip system. Jean, 232-0281, 232-3990
VACATION
TAHOE KEYS, 3-bdrm, 2.5-bth house w/boat dock, mountain view. Bob, 376-2211
FOR FREE
CAT, abandoned at apt. complex, needs loving home, neutered, full-grown, very affectionate, must have no other cats since he has FIV (feline immuno-deficiency virus), healthy now, can easily live to a ripe old age, would be very happy in a home w/children. 223-8808
Please note also:
Manager, Ron Kolb
EDITOR:
Mary Bodvarsson, X4014
mgbodvarsson@lbl.gov
STAFF WRITERS:
Jeffery Kahn, X4019
Diane LaMacchia, X4015
Mike Wooldridge, X6249
Lynn Yarris, X5375
EDITORIAL ASSISTANT:
Brennan Kreller, X6566
PRODUCTION:
Alice Ramirez
FLEA MARKET/CALENDAR:
Mary Padilla, X5771
currents_calendar@lbl.gov
fleamarket@lbl.gov
Public Information Department
LBNL, MS 65 (Bldg. 65B)
One Cyclotron Rd.
Berkeley, CA 94720
Tel: (510) 486-5771
Fax: (510) 486-6641
LBNL is managed by the University of California for the U.S. Department of Energy